Earlier in the normal subjects that take

Earlier studies have been shown an alteration in activity times, changes
happen in sleep period and feeding behavior together influence circadian
control of the endocrine system (16,17).

In our study, secretion pattern cortisol is similar to the diurnal
ryhtem of cortisol seen in previous reports (3,12) with peak levels occurring
at early morning (06.00 p.m.) and sleep deprivation had no significant effect
on this pattern. The results of Scheer et.al (2009) study showed, the cortisol secretion pattern is more influenced by the internal
daily rhythms rather than behavior (fasting-feeding and sleep/wake) cycles (18).

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The study Fumihisa et al. (2013) showed that night
shift (from 0:00 to 8:00) had not effect on circadian rhythm in male nurses. In
addition,  the concentration of
cortisol  in night shift did not differ
from that of the control group (19).  Also
in our study, the average concentration of cortisol
throughout 24 h  was not significantly
higher in the disrupted sleep group than the control group.

In agreement with
our study, a previous study,  did not show a significant circadian rhythm for
acylated ghrelin in  the normal subjects
that take 3 meals in a day (14). It seems
that the nutritional state of the person is effective on ghrelin secretion
pulses. Fasting augmented all parameters of ghrelin pulsatile secretion (20). In contrast to our study, some previous studies have
reported circadian rhythm for ghrelin (13). This can be due to
methodological differences that prevented comparison  this study with other works.

1- the number of blood sampling: In our
study, The number of blood sampling was 6 times during 24 hours. Whereas in other
works were between 24-72  during 24 hours

2- Age and sex. Most previous studies
that reported circadian rhythm for ghrelin have been done in female subjects and
a very large age range (13,21).

3-sleep and sleep
deprivation condition. In most previous studies, effects of sleep on a diurnal
or nocturnal pattern of ghrelin secretion investigated in the laboratory
conditions not mimicking “real life.

In conclusion, Sleep deprivation has no significant
effect on the diurnal pattern of cortisol. In addition, ghrelin does not show a
circadian rhythm.


The small number of subjects and low
number sampling over 24 h are limitations.