Title: Testing For Macromolecules Objective: Introduction Proteins are complex molecules which consists of carbon, oxygen, hydrogen and nitrogen. All proteins are made up of one or more polymers, known as polypeptides. Its monomers are amino acids. Protein can be divided into primary structure, secondary structure, tertiary structure and quaternary structure. It is important for all living things in the world as they form enzymes, antibodies and hormones. Materials and Procedures: Results and Discussions: Test for Carbohydrates ResultStarch suspension When the yellowish-brown iodine was added into the starch suspension, the starch suspension was changed into dark blue.
Potato slide Discussion Starch suspension When the yellowish-brown iodine was added into the starch suspension, the starch suspension was changed into dark blue. This is due to the yellowish-brown iodine changed into dark blue when there is a presence of starch. This shows a positive test for starch. Potato slide Many dark blue spots were visible under the microscope after the slide was irrigated with iodine.The formation of dark blue spots are the result of there is a presence of starch granules in the potato cells. This shows a positive test for starch.
Discussion In the test tube that containing water, no cloudiness was observed Test for nucleic acid Result Discussion When the slide of onion epidermis that was stained with aceto-orcein stain was viewed under the microscope, it was found that each cell contained one red spot. These red spots contained nucleic acid and were actually the nucleus of the cell. Test for protein ResultAfter the boiling tube that containing the egg white and sodium hydroxide solution was heated in the water bath, the egg white which was a white solid was dissolved to form a light yellow solution. The solution was then changed from light yellow to blue to dark purple when the copper sulfate solution was added to the boiling tube. Discussion The colour of the mixture was changed from light yellow to blue then to dark purple after the copper sulfate solution was added and this shows the presence of protein in egg white. This is due to the nitrogen atoms in peptide chain of egg white form a purple complex with copper( ) ion.