To ensure that the experiment is carried out in a safe manner, I must ensure that all the desktops and areas around the experiment are clear, with nothing on the floor so that no one can trip and fall. I must ensure that no hydrogen peroxide is spilt, and in the event of a spillage, I must inform a teacher and clear it up straight away. I must be careful with glassware, and if anything breaks, I must again inform a teacher and clear it away immediately. In this experiment I am going to see what affects the rate of reaction in an enzyme controlled reaction, and how.
To do this I will add hydrogen peroxide to liquidised celery, which contains the enzyme catalase, and measure the reaction rate. The factors I could alter are Temperature The concentration of the enzyme The concentration of the substrate The surface area of the enzyme pH In this experiment I have decided to alter the concentration of the enzyme. This is because the concentration of the enzyme is probably the easiest to measure, and therefore will hopefully be the most accurate. In order to ensure that the experiment is as fair as possible, I must control the other variables.
Therefore I must keep the concentration of the substrate constant all the way through the experiment, as with the temperature. The surface area of the celery will be difficult to control, but I feel by liquidising the celery I can keep it similar enough to maintain a fair test throughout. To ensure that the experiment is fairly accurate and I have enough results to show an overall trend, I will use five different concentrations of the substrate, and repeat each experiment three times. Prediction I predict that as the concentration of the substrate is increased, the rate of reaction will increase in direct proportion.
Therefore, if I were to double the concentration of the substrate, I would expect the rate of reaction to also double. This is because the Collision Theory states that the more particles in a solution, the more likely they are to react, and more quickly. This is because with more particles, there are more collisions, and thus more chance of a successful collision in which the particles react. An enzyme is a protein that acts as a catalyst. A catalyst is a chemical substance that speeds up a reaction but is not used up during the reaction. One enzyme can be used again and again.
There are two types of reactions involving enzymes, anabolic and catabolic. Anabolic reactions are where the enzyme molecule combines two smaller molecules into one larger molecule. Catabolic reactions are the opposite, breaking down a molecule of a substance into two smaller, different molecules. The reaction in this experiment is catabolic, as it is breaking down the hydrogen peroxide into water and oxygen. The word equation for this is: catalase Hydrogen Peroxide Water + Oxygen The symbol equation is: catalase 2H2O2 2H2O + O2 Diagram Step-by-Step Guide.