Background information: Catalysts are substances that speed up the chemical reaction by decreasing the activation energy. In biology catalyst is called biological catalyst, and enzyme is an example of biological catalyst. Enzyme is very important for biochemical reactions, as it helps the reaction to become faster than usual. The reactions with enzymes will react 10 billion times faster than those solutions without enzymes. (clitoncc)The enzymes can only react with one particular reaction, in order for the reaction to react it needed to find the correct enzyme. uab) Rennin is an enzyme produced in the inner lining of the abomasum. Renin will solidify milk and makes it curdle. Curdling of milk is essential for the proper digestion of milk proteins in the stomach. (buzzle) Aim: To analyze the effect of temperature on Renin and to find the best temperature for enzyme activity Hypothesis: Renin works the best in which the rate of reaction is at the highest or optimum, in 40? C Materials: •Bunsen burner •Wire gauze •Thermometer •Dropper •Mortar •Pestle •Crushed ice •Distilled water •Test-tubes •Measuring cylinder •Beaker •Milk •Junket tablet •Test tube holder Stopwatch Procedures: 1. Junket tablet are crushed with the help of mortar and pestle in addition with 10 ml of water. 2. The temperature of water bath is checked accordingly. 3. Other material is prepared, such as: test tubes, milk, thermometers, measuring cylinders and Bunsen burner. 4. 3ml of milk is poured in each test tube (4). 5. Test tubes then are inserted into the water bath. As the milk suited the temperature desired, 1 ml of renin is added to the first 3 test tubes, while the last one is used as a negative control. 6. All of the tubes is examined and record for every minute for 20 minutes. . Steps are repeated for 2 times. Table Results TemperatureTube 1 (trial 1)Tube 2 (trial 2)Tube 1 (trial 1)Tube 2 (trial 2)Average timeRate of reaction 2016-8881/8 3033222. 51/ 2. 5 40111111 5033723. 751/ 3. 74 6036112. 751/ 2. 75 Discussion and Analysis: The experiment was conducted to analyze when will the renin work at its maximum capacity, at what temperature. The milk will be fully clot and form curd as the renin reach its optimum rate. Four different temperatures of water were given to test the problem, 20, 30, 40, 50, 60 degree Celsius. Two trials were done in the experiment.
The milk will work as a substrate and the junket tablet contain renin (enzyme) will work as the biological catalyst. There are some factors that needed to be constant in order to produce a credible result. The concentration and the volume of the milk needed to be constant, the concentration and the volume of the renin needed to be constant and the milk must come from the source, like for example: greenfields. The temperature given needed to be kept constant, otherwise the results will not be accurate. In order to keep it constant and balance, ice is needed by adding it to the water or by giving heat to the water.
The other difficulties to produce a credible result is to determine whether the milk has fully clot or not, because some of the students may wrongly determine whether the milk has fully clot or not. Two trials were done in the experiment, and it was found that some of the groups’ milk-renin mixture didn’t clot. It was caused by the lack of renin concentration took from the mixture of junket tablet and water. There is possibility, when the junket tablet was grind and given water, some of the junket tablet’s powder haven’t actually dissolve with the water so they precipitated at the bottom.
By using dropper, there is a chance that the students only took the upper part of the mixture so that they didn’t get much concentration of renin from the junket tablet as they precipitated at the bottom. Therefore, the results will not be accurate as the concentration of renin was not enough to make the milk clot. Conclusion: Based on the experiment and the table results, it is proved that the results support my hypothesis. The renin also works at its maximum capacity or at its optimum rate at 40 degree Celsius Safety precautions: -Wear gloves (contaminated by renin will make the person itchy) -Wear gloves due the use of heat